Effect of Agitation on Bacterial Aggregates in Pure Cultures and Raw Milk

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Abstract
A comparison of the effects of various mechanical agitation treatments on bacterial aggregates was performed on 8 pure cultures and 27 raw milk samples. Although both syringing and blending produced significant increases in total counts and psychrotroph counts, blending for 2 min gave the greatest increase in count. Use of the direct epifluorescent filter technique (DEFT) confirmed that syringing and blending reduced bacterial clump size to approximately 2 cells. These agitation treatments markedly improved the correlation between DEFT counts and plate counts.