Detection of Water in Lipase-Catalyzed Reactions in Reverse Micelles as Studied by Infrared Spectroscopy
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis in Biocatalysis
- Vol. 3 (4) , 381-383
- https://doi.org/10.3109/10242429008992081
Abstract
The hydrolytic activity of lipolytic enzymes in reverse micelles can be measured continuously with Fourier Transform infrared spectroscopy (FTIR) by following in the region of the OH-stretching band the water consumption during the reaction. This possibility is unique to reverse micellar solutions, because they are optically transparent and because they contain only a limited amount of water.Keywords
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