Experimental evidence for flexible slope of immobilized pH gradients poured under computer control

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Abstract
Recently, Altland and Altland (Clin. Chem.1984,30, 2098–2103) described three alternative devices for pouring immobilized pH gradients (IPGs) based on simultaneous delivery of a density gradient and a non‐density gradient by using high precision burettes under computer control. One of the expected improvements was an unlimited flexibility for the slope of the non‐density gradient (e. g.the pH gradient) which is stabilized by the density gradient. The human plasma proteins prealbumin and apolipoprotein AI and some of their inherited variants are demonstrated by double one‐dimensional electrophoresis in the sequence polyacrylamide gel electrophoresis followed by isoelectric focusing (IEF) in IPGs of varying slopes in the range of pH 4 to 7. It is shown that the patterns obtained follow the expectations from the gradients drawn on the computer. We conclude that the new devices for pouring IPGs can provide a valuable tool for the easy handling of complex separation problems to be solved by IEF.