Antigenic Relationship Between Pythium insidiosum de Cock et al. 1987 and its Synonym Pythium destruens Shipton 1987
- 1 February 1989
- Vol. 32 (2) , 73-77
- https://doi.org/10.1111/j.1439-0507.1989.tb02205.x
Abstract
Summary: Antigens and rabbit-antisera from holotypes of Pythium insidiosumand P. destruens were prepared to elucidate their antigenic relationship. The antigens and rabbit-antisera of P. insidiosum as well as P. destruens used as a reference system showed that both shared three precipitin bands in common. The antigen and rabbit-antisera of P. destruens and P. insidiosum used as a reference system against other strains isolated from humans and animals with pythiosis, also showed three precipitin bands in common. When we used sera taken from horses with proven pythiosis against antigens of P. insidiosum and P. destruens, six common bands were observed. We concluded that the etiologic agent of pythiosis is a single species P. insidiosum, and could be identified by serologic methods. Zusammenfassung: Zur Untersuchung der Antigenverwandtschaft zwischen Pythium insidiosum und P. destruens wurden die Antigene der Holotypen beider Arten sowie homologe Kaninchen-Antiseren präpariert. Die Antigene beider Arten und ihre Antiseren zeigen drei gemeinsame Präzipi-tationsbanden, und benutzt als Referenzsy-stem gegen Stämme, die von Menschen und Tieren mit Pythiosis isoliert worden waren, konnten ebenfalls drei gemeinsame Präzi-pitationsbanden nachgewiesen werden. An Seren von Pferden mit erwiesener Pythiosis wurden sechs gemeinsame Banden gegen Antigene von P. insidiosum und P. destruens beobachtet. Es wird daraus geschlossen, daß das ätiologische Agens der Pythiosis nur eine einzige Art, nämlich P. insidiosum ist, die durch serologische Methoden erfaßt wird.Keywords
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