On the Determination of Lipoperoxides in Animal Tissues.

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Abstract
The determination of lipoperoxides in animal tissues was studied through recovery experiments. Quantities of peroxides up to about 0.1 [mu]equiv./g tissue added during the extraction of liver with chloroform disappeared during the determination. Lung and muscle destroy amounts of peroxides of the same order, whereas with blood and pancreas the recovery is better. Improved recoveries were obtained by adding large amounts of hydrogen peroxide. The principles disturbing the determination of lipoperoxides do not interfere with the thiobarbituric acid reaction.