Improved mass accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of peptides
- 1 November 1994
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Society for Mass Spectrometry
- Vol. 5 (11) , 955-958
- https://doi.org/10.1016/1044-0305(94)80013-8
Abstract
One problem of matrix-assisted laser desorption ionization coupled to time-of-flight mass spectrometry is the moderate mass accuracy that typically can be obtained in routine applications, Here we report improved mass accuracy for peptides, even when low amounts and complex peptide mixtures are used. A new procedure for preparing matrix surfaces is used, and there is no need to mix the matrix with the sample or to add internal standards. Examples are shown with a mass accuracy better than 50 ppm in a peptide mixture. Peptide mapping as well as sequencing by creating “ragged ends” or “ladder sequencing” should benefit especially from the improved mass accuracy.Keywords
This publication has 8 references indexed in Scilit:
- Protein Ladder SequencingScience, 1993
- Rapid identification of proteins by peptide-mass fingerprintingCurrent Biology, 1993
- Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.Proceedings of the National Academy of Sciences, 1993
- Matrix-assisted laser desorption/ionization mass spectrometry of biopolymersAnalytical Chemistry, 1991
- Fast protein sequence determination with matrix‐assisted laser desorption and ionization mass spectrometryRapid Communications in Mass Spectrometry, 1991
- High-accuracy molecular mass determination of proteins using matrix-assisted laser desorption mass spectrometryAnalytical Chemistry, 1990
- Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltonsAnalytical Chemistry, 1988