[3H]dipyridamole binding to nucleoside transporters from guinea-pig and rat lung
- 15 December 1986
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 240 (3) , 879-883
- https://doi.org/10.1042/bj2400879
Abstract
Membranes from guinea-pig lung exhibited high-affinity binding of [3H]dipyridamole, a potent inhibitor of nucleoside transport. Binding (apparent KD 2 nM) was inhibited by the nucleoside-transport inhibitors nitrobenzylthioinosine (NBMPR), dilazep and lidoflazine and by the transported nucleosides uridine and adenosine. In contrast, there was no detectable high-affinity binding of [3H]dipyridamole to lung membranes from the rat, a species whose nucleoside transporters exhibit a low sensitivity to dipyridamole inhibition Bmax values for high-affinity binding of [3H]dipyridamole and [3H]NBMPR to guinea-pig membranes were similar, suggesting that these structurally unrelated ligands bind to the NBMPR-sensitive nucleoside transporter with the same stoichometry.This publication has 19 references indexed in Scilit:
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