Factors Affecting Factor XIII Assay by Dansyl Cadaverine Incorporation

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Abstract
Factors affecting the activation of factor XIII and its subsequent action in incorporating the fluorescent amine, dansyl cadaverine, into casein have been investigated. A comparison of the activation of factor XIII at 30° C and 37° C using 5 NIH units Maws bovine thrombin showed that increasing the temperature shortened the time required for maximal activity from 4-10 minutes to 4 minutes. A sharper peak of activity was observed at the higher temperature and a more rapid fall with increasing activation time. At 30° C, 25 units Parke Davis thrombin topical gave similar results to 5 units Maws thrombin topical. When an activation period of 10 minutes was selected, 5 units of thrombin gave the highest values of factor XIII activity. The optimal level of calcium in the incubation medium was found to be 12 mM.

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