DEVELOPMENT AND APPLICATION OF A HETEROLOGOUS RADIOIMMUNOASSAY FOR OVINE FOLLICLE-STIMULATING HORMONE

1 July 1976

journal article
research article
Published by Bioscientifica in Journal of Endocrinology

Vol. 70 (1) , 69-79
https://doi.org/10.1677/joe.0.0700069

Abstract

SUMMARY: A highly specific and sensitive heterologous double antibody radioimmunoassay for ovine follicle-stimulating hormone (oFSH) is described in detail. The assay using a rabbit antiserum to human FSH and either ¹²⁵I-labelled rat FSH or ¹²⁵I-labelled oFSH as tracer is specific for FSH. A maximum cross-reaction (B/Bo = 50%) of 0·1% was observed with other ovine, rat or human pituitary hormones or human chorionic gonadotrophin. Serum levels of oFSH in samples collected daily throughout the oestrous cycle showed large individual variations. In five out of nine animals a peak of FSH was observed on the day of oestrus.

This publication has 3 references indexed in Scilit:

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Archives of Biochemistry and Biophysics, 1967
Purification and Characterization of Follicle-Stimulating Hormone from Sheep Pituitary Glands^*
Biochemistry, 1966
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