STUDIES ON MONOCYTES IN INFLAMMATORY BOWEL-DISEASE - FACTORS INFLUENCING MONOCYTE LYSOSOMAL ENZYME-ACTIVITY

  • 1 January 1980
    • journal article
    • research article
    • Vol. 39  (3) , 785-791
Abstract
The effect of endotoxin and immune complexes on monocyte lysosomal enzyme activity was studied in patients with Crohn''s disease and ulcerative colitis. Immune complexes made at equivalence or in antigen excess caused a rapid fall in intracellular activity of the enzyme N acetyl-.beta.-D-glucosaminidase which was significantly greater than that seen when cells were incubated with medium alone. Endotoxin had no effect on intracellular enzyme activity but there was a significant elevation of activity in the supernatants. Cells from patients with inflammatory bowel disease were no different to cells from healthy control subjects in their response to endotoxin or immune complexes.