A Simple Procedure for the Purification of Bovine Fibrin from Clotted Blood by the Use of Detergents
- 1 January 1980
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 10 (1) , 43-57
- https://doi.org/10.1080/00327488008061718
Abstract
A rapid and simple procedure for the preparation, directly from clotted whole blood, of bovine fibrin free of cell membrane contaminants is described. The procedure exploits the ability of detergents to solubilize and extract cell membrane proteins from homogenized clot fragments. The non-denaturing detergents, TX-100∗ and DOC, used in sequence, or the denaturing detergent, SDS, used alone, proved equally effective. The resulting fibrin has the solubility properties and the subunit composition on SDS gels of highly cross-linked fibrin.This publication has 10 references indexed in Scilit:
- Ultrastructural and biochemical analyses of isolated basement membranes from kidney glomeruli and tubules and brain and retinal microvesselsJournal of Ultrastructure Research, 1978
- Studies on an activator of the (Ca2++Mg2+)-ATPAse of human erythrocyte membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- A simple, versatile, nondisruptive method for the isolation of morphologically and chemically pure basement membranes from several tissuesLife Sciences, 1975
- THE ORGANIZATION OF PROTEINS IN THE HUMAN RED BLOOD CELL MEMBRANEThe Journal of cell biology, 1974
- Maturation of the head of bacteriophage T4Journal of Molecular Biology, 1973
- Structural Aspects of the Fibrinogen to Fibrin ConversionAdvances in Protein Chemistry, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Binding of Dodecyl Sulfate to Proteins at High Binding Ratios. Possible Implications for the State of Proteins in Biological MembranesProceedings of the National Academy of Sciences, 1970
- Limitations of Tritium Measurements by Liquid Scintillation Counting of Emulsions.Analytical Chemistry, 1966
- A rapid method for the quantitative assay of proteolytic enzymesAnalytical Biochemistry, 1961