Isolation of pterocarpan synthase, the terminal enzyme of pterocarpan phytoalexin biosynthesis in cell suspension cultures of Cicer arietinum
- 1 August 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 235 (1-2) , 47-50
- https://doi.org/10.1016/0014-5793(88)81231-6
Abstract
Chickpea (Cicer arietinum L.) cell suspension cultures transferred into a medium containing yeast extract as elicitor accumulate the phytoalexins medicarpin and maackiain. Concomitant with the accumulation of the pterocarpans, a new enzyme activity is induced which catalyses the conversion of the 2′‐hydroxyisoflavanone vestitone to the pterocarpan medicarpin. Maximum enzyme activity was reached 12 h after transfer of cells and then activity declined. The soluble enzyme was partially purified and characterized as pterocarpan synthase.Keywords
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