Immediate GTP hydrolysis upon FtsZ polymerization
- 1 March 2002
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 43 (6) , 1517-1521
- https://doi.org/10.1046/j.1365-2958.2002.02828.x
Abstract
Summary: To understand the polymerization dynamics of FtsZ, a bacterial cell division protein similar to tubulin, insight is required into the nature of the nucleotide bound to the polymerized protein. In a previous study, we showed that the FtsZ polymers contain mostly GDP. A recent study challenged this result, suggesting that the polymerized FtsZ is in a GTP‐bound state. Here, we show that, when radiolabelled [γ‐32P]‐GTP is used to polymerize FtsZ, GTP is hydrolysed instantaneously. The FtsZ polymer contains both GDP and the radiolabelled inorganic phosphate.Keywords
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