LE DOSAGE DE L'ADRÉNALINE ET DE LA NORADRÉNALINE : I. RÉACTION MODIFIÉE DES CATÉCHOLAMINES AVEC L'ÉTHYLÈNEDIAMINE

Publisher Website
Google Scholar
Abstract
A modification to the method of Weil-Malherbe and Bone for the estimation of adrenaline and noradrenaline is suggested. The catecholamines are first allowed to react with ammonium molybdate at room temperature. Ethylenediamine reagent is then added to the catecholamine–molybdate complexes, and the reaction is carried out in two different steps: at room temperature, and at 50 °C. This procedure ensures a more adequate oxidation of the catecholamines and controls the variable effect of metallic ions either present in biologic samples or introduced during adsorption on alumina. The fluorescence is also significantly increased, although the amount of ethylenediamine added is reduced, thus decreasing the fluorescence of the reagent blanks. Another modification of the original method for the differential estimation of both catecholamines is based solely on the different rates of reaction of the molybdate complexes with ethylenediamine; this eliminates the need of reading the fluorescence at two different wavelengths.

This publication has 0 references indexed in Scilit: