FATE OF INTRATRACHEALLY INSTALLED BENZO(A)PYRENE IN ISOLATED PERFUSED RAT LUNG OF BOTH CONTROL AND 20-METHYLCHOLANTHRENE PRETREATED RATS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 13  (2) , 259-271
Abstract
The fate of intratracheally installed carcinogenic 3H-benzo(a)pyrene in the isolated perfused lung of control and 20-methylcholanthrene pretreated rats and in perfusion fluid was studied. The covalent binding of benzo(a)pyrene metabolites in the lung tissue itself was greatly enhanced by 20-methylcholanthrene pretreatment of rats. The appearance of unchanged 3H-benzo(a)pyrene in the perfusion fluid of 20-methylcholanthrene-lung was decreased as compared to control lung perfusion. This was accompanied by the increase of water-soluble metabolites of benzo(a)pyrene in the perfusion fluid of 20-methylcholanthrene-lung. The metabolite profile of benzo(a)pyrene in the lungs showed that the phenols (7-fold) and 9,10-diols (5-fold) were especially increased.