A triple-resonance pulse scheme for selectively correlating amide1HN and15N nuclei with the1Hα proton of the preceding residue
- 1 July 1992
- journal article
- Published by Springer Nature in Journal of Biomolecular NMR
- Vol. 2 (4) , 389-394
- https://doi.org/10.1007/bf01874816
Abstract
A 3D1H−15N−13C triple resonance experiment is presented that contains exclusively cross peaks between the1HN and15N nuclei of one residue with the Hα of the preceding residue. The pulse sequence, designed to minimize the time coherence, is transverse on nuclei with short T2 values. The experiment consists of coherence transfers via one-bond couplings from the HN via N, CO, Cα to the Hα and back to the HN for detection; it is called HN(COCA)HA. The experiment was tested on uniformly15N- and13C-enriched T4 lysozyme.Keywords
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