Label-fracture of cell surfaces by replica staining.
Open Access
- 1 November 1988
- journal article
- abstracts
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 36 (11) , 1413-1418
- https://doi.org/10.1177/36.11.2459187
Abstract
We introduce replica-staining label-fracture, a method for the cytochemical mapping of membrane surfaces. This method is a corollary of the rationale of label-fracture (Pinto da Silva and Kan, 1984: J Cell Biol 99:1156). After freeze-fracture the exoplasmic halves of the membrane remain attached to the replica. We show that cytochemical labeling of cell surfaces can be performed by direct post-fracture staining of freeze-fracture replicas. This new variant of label-fracture leads to miniaturization of labeling procedures and allows standardization of labeling conditions and simultaneous processing of different specimens.Keywords
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