RNA phosphorylation: a polynucleotide kinase function in mouse L cell nuclei

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Abstract
Analysis of [.gamma.-32P]ATP in vitro labeled nuclear RNA showed transfer of .gamma.-32P from ATP to form the 5''-terminal monophosphate for large RNA molecules. This finding in an actively transcribing nuclear system capable of guanylation and methylation reactions indicated that polynucleotide kinase activity in the eukaryotic nucleus might be functional in kinase reactions involving RNA. It further suggested a participation in the posttranscriptional modification reactions involved in RNA processing. All 4 nucleosides were found to act as acceptors at the 5'' termini of RNA. It was also shown that both ATP and GTP could serve as donors in the nuclear polynucleotide kinase reaction.