New Procedures for Rearing Cabbage Loopers and Other Lepidopterous Larvae for Propagation of Nuclear Polyhedrosis Viruses

Abstract
Paraffin-coated paper bags were used successfully to rear Trichoplusia ni (Hübner) for use as hosts of entomogenous viruses. They also reduced the cost of producing 100 pupae at least 10-fold and may make it easier to handle the pupae. Three new diets were acceptable, and one drastically reduced the cost of the rearing medium. The new techniques greatly reduced the cost of producing a nuclear polyhedrosis virus isolated from Autographa californica (Speyer) using larvae of T. ni or Spodoptera exigua (Hübner).

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