Incorporation of two nonnatural amino acids into proteins through extension of the genetic code
Open Access
- 1 November 1999
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Symposium Series
- Vol. 42 (1) , 79-80
- https://doi.org/10.1093/nass/42.1.79
Abstract
A novel method of the in vitro incorporation of two nonnatural amino acids into proteins through extension of the genetic code was developed. The streptavidin mRNA containing AGGU and CGGG, and chemically aminoacylated tRNAACCU and tRNACCCG were prepared, then they were added into E. coli in vitro protein synthesizing system. As a result, two nonnatural amino acids were successfully incorporated into desired sites of streptavidin.Keywords
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