Laboratory Studies of Transovarial Transmission of Trivittatus Virus by Aedes Trivittatus *

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Abstract
Aedes trivittatus were experimentally exposed through a membrane to a concentration of trivittatus (TVT) virus in dog defibrinated blood of 103.4–103.5 SMICLD50/0.025 ml. Transovarial and transstadial transmission was demonstrated. Fifty-five percent of the infected mosquitoes tested transovarially transmitted TVT virus. TVT virus was isolated from 16% of the F1 generation progeny examined, including 19% of the larvae, 23% of adult females, and 10% of adult males.