ISOLATION AND IDENTIFICATION OF 3α,17α,21-TRIHYDROXY-5α-PREGNAN-20-ONE (ALLO-TETRAHYDRO-COMPOUND S) AFTER ADMINISTRATION OF 3β, 17α,21-TRIHYDROXYPREGN-5-EN-20-ONE(17α,21-DIHYDROXYPREGNENOLONE)

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Abstract
3[alpha],17[alpha],21-Trihydroxy-5[alpha]-pregnan-20-one (allo-tetrahydro-compound S) has been isolated and identified in human urine after the administration of 3[beta],17[alpha],21-trihydroxypregn-5-en-20one (17[alpha],21-dihydroxypregnenolone) to a normal subject. Its identification was established on the basis of chromatography in four different systems and in 2 systems after acetylation. Its oxidation product was proved to be androsterone by chromatography of the free compound and of the acetate. Of this steroid 60% was found in the leastpolar ester sulfate fraction and 40% in the glucuronide fraction.