Collection of peripheral blood mononuclear cells as a byproduct of plateletpheresis with two different blood cell separators

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Abstract
Peripheral blood mononuclear cells (PBMC) were collected as a byproduct of plateletpheresis of normal blood cell donors using modifications to standard automated protocols on either the CS‐3000 or Spectra blood cell separator machine. Comparison of the PBMC products obtained showed X ± SD WBC yields of 5.3 ± 3.4 vs. 3.8 ± 2.0 × 109 with the CS‐3000 and Spectra, respectively (P < .0001). The majority of the cells were lymphocytes, with 13–15% monocytes with both machines. Sixteen percent of the WBC collected with the Spectra, but only 1% of those collected with the CS‐3000, were granulocytes. The CS‐3000 PBMC product contained fewer RBC (0.2 ± 0.1 × 1011 vs. 2.4 ± 0.6 × 1011) and more platelets (1.6 ± 0.6 × 1011 vs. 0.35 ± 0.39 × 1011) in a smaller volume (40 ± 14 ml vs. 229 ± 37 ml) than the Spectra products. Comparison of the platelet collections harvested when PBMC were also collected to platelets harvested using standard procedures on the same machine showed no change in platelet. WBC, or RBC yields for the Spectra. A significant increase in mean WBC contamination from 40 ± 56 × 107 to 112 ± 205 × 107 and a small, but statistically insignificant, decrease in platelet yield from 4.1 ± 1.2 × 1011 to 3.9 ± 1.8 × 1011 was observed in the CS‐3000 platelet collections when PBMC were harvested. There was no sustained change in donor lymphocyte counts and no change in acute donor side effects or time requirements when PBMC were collected. The procedural modifications were easily learned by multiple operators, and required about 15 and 5 min additional operator time on the CS‐3000 and Spectra, respectively. Thus, harvest of large numbers of PBMC as a byproduct of plateletpheresis can be readily accomplished with either of 2 different blood cell separator machines. The cells obtained have been useful for a variety of purposes in both clinical hospital and basic research laboratories.