Positional Isotope Exchang
- 1 January 1988
- journal article
- review article
- Published by Taylor & Francis in Critical Reviews in Biochemistry
- Vol. 23 (1) , 1-26
- https://doi.org/10.3109/10409238809103118
Abstract
The detection of intermediates in enzyme-catalyzed reactions can be accomplished by several techniques. For those intermediates which do not have easily observed electronic spectra, use can be made of isotope exchange phenomena if the chemistry of the reaction is appropriate. Recently, the technique of positional isotope exchange (intramolecular isotopic scrambling) has been used to study several reactions which have been thought to involve high-energy intermediates in their mechanisms. A review of some of these reactions and the limitations of the method are presented in this article.Keywords
This publication has 28 references indexed in Scilit:
- Measurement of positional isotope exchange rates in enzyme-catalyzed reactions by fast atom bombardment mass spectrometry: application to argininosuccinate synthetaseBiochemistry, 1985
- A positional isotope exchange study of the argininosuccinate lyase reactionBiochemistry, 1984
- Enzyme-catalyzed positional isotope exchange by phosphorus-31 nuclear magnetic resonance spectroscopy using either oxygen-18- or oxygen-17-.beta.,.gamma.-bridge-labeled adenosine 5'-triphosphateJournal of the American Chemical Society, 1983
- A method for determining the positional isotope exchange in a nucleoside triphosphate: cyclization of nucleoside triphosphate by dicyclohexylcarbodiimideBiochemistry, 1980
- Phosphorus-31 nuclear magnetic resonance application to positional isotope exchange reactions catalyzed by Escherichia coli carbamoyl-phosphate synthetase: analysis of forward and reverse enzymic reactionsBiochemistry, 1980
- Use of phosphorus-31 nuclear magnetic resonance to distinguish bridge and nonbridge oxygens of oxygen-17-enriched nucleoside triphosphates. Stereochemistry of acetate activation by acetyl coenzyme A synthetaseBiochemistry, 1979
- Kinetic mechanism of Escherichia coli carbamoyl-phosphate synthetaseBiochemistry, 1978
- Isotopic ( 18 O) shift in 31 P nuclear magnetic resonance applied to a study of enzyme-catalyzed phosphate—phosphate exchange and phosphate (oxygen)—water exchange reactionsProceedings of the National Academy of Sciences, 1978
- Partition analysis and concept of net rate constants as tools in enzyme kineticsBiochemistry, 1975
- Purification and Specific Kinetic Properties of Erythrocyte Uridine Diphosphate Glucose PyrophosphorylaseJournal of Biological Chemistry, 1969