Preservation of the sheath of a blue-green alga, Anabaena flos-aquae A-37

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Abstract
The preservation of the sheath of Anabaena flos-aquae A-37 was achieved when the growing culture was pretreated with a low concentration of glutaraldehyde and fixed using the standard Kellenberger method. The thin sections were stained with an aqueous solution of uranyl acetate at an elevated temperature. The sheath of A. flos-aquae A-37 consisted of fibrous structures and measured about 1.0–2.0 nm in diameter. The fibers were parallel with one another and to the cell surface. The method used eliminated the use of organic dyes and provided an excellent visualization of the sheath and the internal organization.

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