The Glycosyltransferase Gene Encoding the Enzyme Catalyzing the First Step of Mycothiol Biosynthesis ( mshA )
Open Access
- 1 June 2003
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 185 (11) , 3476-3479
- https://doi.org/10.1128/jb.185.11.3476-3479.2003
Abstract
Mycothiol is the major thiol present in most actinomycetes and is produced from the pseudodisaccharide 1 d - myo -inosityl 2-acetamido-2-deoxy-α- d -glucopyranoside (GlcNAc-Ins). A transposon mutant of Mycobacterium smegmatis shown to be GlcNAc-Ins and mycothiol deficient was sequenced to identify a putative glycosyltransferase gene designated mshA . The ortholog in Mycobacterium tuberculosis , Rv0486, was used to complement the mutant phenotype.Keywords
This publication has 22 references indexed in Scilit:
- Mycothiol-Deficient Mycobacterium smegmatis Mutants Are Hypersensitive to Alkylating Agents, Free Radicals, and AntibioticsAntimicrobial Agents and Chemotherapy, 2002
- ATP-Dependent l-Cysteine:1d-myo-Inosityl 2-Amino-2-deoxy-α-d-glucopyranoside Ligase, Mycothiol Biosynthesis Enzyme MshC, Is Related to Class I Cysteinyl-tRNA SynthetasesBiochemistry, 2002
- Sucrose metabolism: Anabaena sucrose‐phosphate synthase and sucrose‐phosphate phosphatase define minimal functional domains shuffled during evolutionFEBS Letters, 2002
- N -Acetyl-1- d - myo -Inosityl-2-Amino-2-Deoxy-α- d -Glucopyranoside Deacetylase (MshB) Is a Key Enzyme in Mycothiol BiosynthesisJournal of Bacteriology, 2000
- Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesisCurrent Opinion in Chemical Biology, 2000
- Identification of Essential Amino Acids in the Bacterial α-Mannosyltransferase AceAJournal of Biological Chemistry, 2000
- The pimB Gene of Mycobacterium tuberculosis Encodes a Mannosyltransferase Involved in Lipoarabinomannan BiosynthesisPublished by Elsevier ,1999
- Characterization ofMycobacterium smegmatisMutants Defective in 1-d-myo-Inosityl-2-amino-2-deoxy-α-d-glucopyranoside and Mycothiol BiosynthesisBiochemical and Biophysical Research Communications, 1999
- PROSPECTS FOR NEW INTERVENTIONS IN THE TREATMENT AND PREVENTION OF MYCOBACTERIAL DISEASEAnnual Review of Microbiology, 1995
- Thiols of Intracellular PathogensEuropean Journal of Biochemistry, 1994