Cultivation In Vitro of Cells Derived From Adult C3H Mouse Ventral Prostate2

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Abstract
Cell lines have been established by the dispersion of organ cultures of adult C3H mouse ventral prostates. Seven lines were obtained, by use of Eagle's basal essential medium (BME) plus fetal calf serum, and one morphologically different line was developed in NCTC 109 medium plus fetal calf serum. The effect of plating the cells at different densities in the primary culture was studied. The cell lines obtained in BME medium did not pile up in cultures before transformation, did not grow in 1% fetal calf serum, and were aneuploid. Spontaneous malignant transformation occurred in 3 of 8 cell lines and was correlated with piling up in the culture. However, these spontaneous malignant transformations were delayed, occurring only at 261, 344, and 570 days in culture. These cell lines, usually after about 100 days' passage when spontaneous malignant transformation has not been detected, can undergo malignant transformation on treatment with carcinogenic hydrocarbons.

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