Studies on the plasma membrane of normal and psoriatic keratinocytes. 4. Characterization of glycoconjugates

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Abstract
A substantial proportion (20-50%) of radioactive sugar incorporated into glycoconjugates by normal human keratinocytes was soluble in chloroform-methanol; using (14C)-galactose as precursor .apprx. 1/2 this fraction was neutral lipid. The incorporation of labeled sugars into the lipid fraction was consistently increased in keratinocytes derived from psoriatic lesions. The abnormality in fucose metabolism was confirmed. In particular the MW of fucosylated glycopeptides appears to be abnormally high in the untreated psoriatic lesion, possibly reflecting an increased degree of branching. In psoriatic uninvolved epidermis and in treated psoriatic lesions the situation is reversed, the MW being significantly lower than normal.