Demonstration of an iron-siderophore-binding protein in the outer membrane of Pseudomonas aeruginosa
- 1 May 1983
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 40 (2) , 665-669
- https://doi.org/10.1128/iai.40.2.665-669.1983
Abstract
Outer membranes prepared from Pseudomonas aeruginosa grown in low-iron medium bind three times as much [59Fe]pyochelin as do membranes from cells grown in high-iron medium. The deletion of pyochelin reduced 59Fe binding to background levels. Autoradiographic analysis of sodium dodecyl sulfate polyacrylamide gel electrophoretograms of outer membrane preparations previously incubated with [59Fe]pyochelin revealed that the iron-siderophore complex bound to a low-molecular-weight protein of approximately 14,000.This publication has 25 references indexed in Scilit:
- Properties and proteolysis of ferric enterobactin outer membrane receptor in Escherichia coli K12Biochemistry, 1982
- Iron uptake with ferripyochelin and ferric citrate by Pseudomonas aeruginosaJournal of Bacteriology, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Chromosomal genetics of Pseudomonas.1979
- The critical role of iron in host-bacterial interactions.Journal of Clinical Investigation, 1978
- Ferric enterobactin transport system in Escherichia coli K-12. Extraction, assay, and specificity of the outer membrane receptorBiochemistry, 1978
- The role of colicin receptors in the uptake of ferrienterochelin by Escherichia coli K-12Biochemical and Biophysical Research Communications, 1977
- Iron and Susceptibility to Infectious DiseaseScience, 1974
- Membrane D-lactate dehydrogenase from Escherichia coli. Purification and propertiesBiochemistry, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970