Detection of Plasminogen Activator in Macrophage Culture Supernatants by a Photometric Assay

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Abstract
Plasminogen activator is usually detected indirectly through the lysis of radioiodinated fibrin. It was investigated whether the fibrinolysis test may be substituted by a photometric assay using a synthetic chromogenic substrate (H-D-Val-Leu-Lys-p-nitroanilide; S-2251). Supernatants from cultured murine macrophages served as a source of plasminogen activator. Under standard conditions the photometric assay is about 2 times as fast and 2- to 4-fold more sensitive than the fibrinolysis assay. Reproducibility of the photometric test was within 1.5-3.5% SD compared to the fibrinolysis test which was found between 10-20% SD.