Inactivation of Staphylococcal Thermonuclease by an Enzyme-Like Factor Produced by Streptococcus faecalis subsp. liquefaciens

Abstract
The thermonuclease of Staphylococcus aureus was inactivated during incubation with filtrates from cultures of Streptococcus faecalis subsp. liquefaciens. Of 53 isolates, 40 produced a thermonuclease-inactivating factor (TIF). The TIF was heat-labile, non-dialyzable and optimally active at 55 C and pH 7.0. These characteristics suggest TIF is a proteolytic enzyme. The common occurrence of TIF in cultures of S. faecalis subsp. liquefaciens may limit the value of the thermonuclease test for foods containing large populations of this organism.

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