Characterization of a New Picorna-like Virus Isolated from Aphids

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Abstract
Summary A new picorna-like virus, provisionally named aphid lethal paralysis virus (ALPV), was isolated from the aphid Rhopalosiphum padi. The virus particles are isometric with a diameter of 26 nm, a sedimentation coefficient of 164S and a density in caesium chloride of 1.34 g/ml. Virions contain a 9.7 kb polyadenylated, ssRNA and four polypeptides: three major polypeptides of M r 34400, 32000 and 31200 and one minor polypeptide of M r 40800. ALPV is serologically unrelated to R. padi virus of aphids but is serologically related to cricket paralysis virus. The properties of this virus indicate that it should be classified in the family Picornaviridae.

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