Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays
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Open Access
- 1 July 2005
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 71 (7) , 4117-4120
- https://doi.org/10.1128/aem.71.7.4117-4120.2005
Abstract
Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community structure.Keywords
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