The quantitative estimation of vitamin D

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Abstract
An examination of the fecal pH of rats on a rachitogenic diet, to which supplementary substances are fed in known amounts, indicates the presence or absence of vitamin D, as shown by the authors in a previous paper (Biochem. Jour. 20, 1351, 1926); definite acidification of the feces occurs in the presence of the vitamin. The authors now show that the change in pH manifested by the feces of animals on the basal diet is, within certain limits, proportional to the quantity of vitamin D fed as a supplementary ration.

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