The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Products of complete digestion with pancreatic ribonuclease and ribonuclease T1
- 1 February 1976
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 153 (2) , 437-446
- https://doi.org/10.1042/bj1530437
Abstract
1. The nucleotide chain of tRNA Cys from baker's yeast was readily split at the anticolon into two large fragments by brief treatment with ribonuclease T1.2. The whole molecule and the two derived large fragments were completely digested with (a) pancreatic ribonuclease and (b) ribonuclease T1. The fragments present in each of the digests were separated and sequenced by conventional methods. 3. The groups of fragments derived from the two methods of digestion were entirely compatible with each other. 4. The molecule is 75 nucleotides long, but, as isolated, lacks the terminal adenosine and the neighboring cytidylic acid residue. The minor nucleotides 1-methyladenylic acid, 7-methylguanylic acid, 5-methylcytidylic acid and N6 (γγ-dimethylallyl)adenylic acid (isopentenyladenylic acid) were identified.This publication has 11 references indexed in Scilit:
- The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Identification of the products from partial degradation of the molecule and derivation of the complete sequenceBiochemical Journal, 1976
- Partial degradation of transfer RNAs and transfer RNA fragments by spleen phosphodiesterase as studied by disc electrophoretic methodsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Complete Digestion of Tyrosine Transfer Ribonucleic Acid from Torulopsis utilis with Pancreatic and T1 RibonucleasesThe Journal of Biochemistry, 1972
- Chromatographic separation of mononucleotides derived from transfer ribonucleic acidsBiochemical Journal, 1971
- Isolation of cytokinins from tRNABiochemical and Biophysical Research Communications, 1969
- On the Specificity of the Reduction of Transfer Ribonucleic Acids with Sodium BorohydrideEuropean Journal of Biochemistry, 1969
- Cytokinins: Isolation and identification of 6-(3-methyl-2-butenylamino)-9-β-D-ribofuranosylpurine (2iPA) from yeast cysteine tRNABiochemical and Biophysical Research Communications, 1969
- Effect of Chemical Modification of Dihydrouridine in Yeast Transfer Ribonucleic Acid on Amino Acid Acceptor Activity and Ribosomal BindingJournal of Biological Chemistry, 1968
- Oligonucleotides from Yeast Tyrosine Transfer Ribonucleic AcidJournal of Biological Chemistry, 1967
- Large Oligonucleotides Isolated from Yeast Tyrosine Transfer Ribonucleic Acid after Partial Digestion with Ribonuclease T1Journal of Biological Chemistry, 1967