AUTOMATIC SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES WITH TERMINAL PHOSPHATE GROUPS

  • 1 August 1988
    • journal article
    • research article
    • Vol. 14  (8) , 1034-1039
Abstract
3''- and 5''-phosphorylated oligodeoxyribonucleotides have been synthesized on the "Victoria-4M" automatic synthesizer by phosphoramidite method. Two approaches have been suggested: introduction of a terminal ribo-unit as a potential source of phosphate group or the use of hydroxyl-containing polymer supports which loose the end product of the synthesis via .beta.-elimination reaction. Yields of oligonucleotides obtained according to both schemes proved to be almost identical. Using the first approach, oligonucleotides containing units with altered configuration of the sugar (xylo-thymidine and arabino-uridine) have been obtained.

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