ELECTROPHORETIC ANALYSIS OF HLA‐DR2 MOLECULES ISOLATED FROM HLA‐Dw2 AND HLA‐Dw 12 CELL LINES

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Abstract
To answer the question of whether or not polymorphism exists among HLA‐DR2 molecules derived from cells homozygous for HLA‐DR2, but expressing different HLA‐D specificities, HLA‐DR2 molecules were isolated from HLA‐Dw2 and HLA‐Dw12 homozygous cells using a monoclonal antibody operationally monospecific for HLA‐DR2, and were compared to each other by two‐dimensional gel eletrophoresis. No electrophoretically discernible polymorphism was found in either the heavy or the light chain subunits of the HLA‐DR2 molecules. These findings are in marked contrast with previous observations that each of the HLA‐DR4‐associated HLA‐D clusters expresses an electrophoretically distinct HLA‐DR4 light chain.