INVITRO ACTIVITY OF COTRIMOXAZOLE ON INTRACELLULAR MULTIPLICATION OF TOXOPLASMA-GONDII

  • 1 January 1975
    • journal article
    • research article
    • Vol. 10  (4) , 307-315
Abstract
Co-trimoxazole, from 60 .mu.g/ml, inhibited the replication of T. gondii within Hela cells and mouse peritoneal macrophages. The percentage of inhibition reached practically 100% after 18 h of treatment at 37.degree. C. More prolonged treatment resulted in an eradication of the organisms from the cell monolayers. Similar doses of spiramycin were quite ineffective against intracellular toxoplasma. The active doses of co-trimoxazole used were not harmful for cell cultures. Co-trimoxazole also destroyed clones (rosaces) of toxoplasma which were formed during the past 18 of intracellular replication in the absence of the drug. Trimethoprim was the only effective compound of cotrimoxazole on intracellular parasites; the adjunction of sulfamethoxazole produced a marked synergistic effect. The present findings confirm the great efficiency of co-trimoxazole in the treatment of experimental toxoplasmosis in mice performed previously in this laboratory.