Distribution of .ALPHA.1- and .ALPHA.2-adrenoceptors in brush border and basolateral membranes from rat kidney cortical tubules.
- 1 January 1987
- journal article
- research article
- Published by Elsevier in The Japanese Journal of Pharmacology
- Vol. 45 (2) , 284-287
- https://doi.org/10.1254/jjp.45.284
Abstract
To examine the localization of .alpha.-adrenoceptors within the kidney tubule, binding experiments with 125 I-HEAT and 3H-yohimbine were performed using cortical homogenates, basolateral membranes (BLM) and brush border membranes (BBM). Bmax values for 125I-HEAT or 3H-yohimbine binding were 2.3- or 2.0-fold that of the homogenate in BBM and 8.2- or 10.7-fold in BLM, respectively. Kd values for 125I-HEAT or 3H-yohimbine were not significantly different between BBM and BLM. These results indicate that both types of .alpha.-adrenoceptors seem to be localized largely in the BLM.This publication has 3 references indexed in Scilit:
- Alpha2-adrenoceptors and cellular cAMP levels in single nephron segments from the ratAmerican Journal of Physiology-Renal Physiology, 1985
- Distribution of alpha-adrenergic receptors in the rabbit nephron.The Tohoku Journal of Experimental Medicine, 1984
- Localisation of [3H]clonidine binding to membranes from guinea pig renal tubulesBiochemical Pharmacology, 1982