Improved Synthesis of CMP-Sialates Using Enzymes from Frog Liver and Equine Submandibular Gland

Publisher Website
Google Scholar
Abstract
An efficient method for the preparation of CMP-N-acetylneuraminic acid using crude or partially purified CTP:N-acylneuraminate cytidylyltransferase [EC 2.7.7.43] from equine submandibular gland or frog liver is described. The yield of the sugar nucleotide after purification by ion-exchange chromatography and gel filtration was 95%. The compound was studied by 360 MHz 1H-NMR spectroscopy in addition to the usual chemical and physical analyses. The preparation of radioactive or unlabeled CMP-4-O-methyl-N-acetyl-neuraminic acid, which is not known to occur in nature, was achieved in 17% yield with the aid of the equine enzyme.