Salt induced synthesis of new proteins in the roots of rice varieties

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Abstract
Ten‐day‐old seedlings of rice (Oryza sativa L., varieties c.v. (Nona bokra, Basmati, IR28, and IR29) grown on MS medium were used in this study. Total protein extract from roots of control and 2% salt‐treated plants were analyzed by two dimensional electrophoresis. Following eight hours of salt stress including terminal three hours of in vivo labeling, two new proteins of molecular weights corresponding to 27 and 25.5 kD were detected. These proteins were not detectable with silver staining after one day of stress but became evident after two days of salt treatment and gradually increased up to four days. During recovery after two days of treatment, their levels decreased gradually and were present even after two days of recovery, though, in decreased amounts. No such protein was induced in plants stressed with copper (Cu), cadmium (Cd), heat, ABA, or PEG treatments. Quantitative changes in the amount of other proteins were also observed. This indicates that the rice plant may adjust to the changes in its ionic environment by altering its macromolecular composition of root tissue.