Effects of gold compounds on function of phagocytic cells

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Abstract
The effect of sodium aurothiomalate and auranofin on the generation of superoxide anions (O 2 − ) by polymorphonuclear leukocytes (PMNLs) and adherent mononuclear phagocytic cells (AMNCs) has been investigated. Sodium aurothiomalate at final concentrations of 1, 10, and 100 μg Au/ml and auranofin ranging from 0.1 to 2.0 μg Au/ml were used in the reactions involving all celt types. Results have been compared between cells drawn from normal controls and patients with active rheumatoid disease. The effect of gold compounds on both cell types was assessed following activation by phorbyl myristate acetate (1×10−8 M) and N-formyl-methionylleucyl-phenylalanine (1×10−4 M) using a cytochromec reduction method. Sodium aurothiomalate at the maximum concentration modestly inhibited O 2 − generation by PMNLs but not AMNCs. Auranofin inhibits O 2 − generation by both cell types. Inhibition of cells from patients with rheumatoid arthritis was greater than that seen with cells from normal controls.