A Novel Promoter Sequence Is Involved in the Oxidative Stress-Induced Expression of the Adult T-Cell Leukemia-Derived Factor (ADF)/Human Thioredoxin (Trx) Gene

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Abstract
Adult T cell leukemia-derived factor (ADF) is a human thioredoxin (Trx) and is a disulfide reducing protein with various biological functions. We found that expression of the ADF/Trx gene was increased by oxidative agents such as hydrogen peroxide, diamide and menadione in Jurkat cells. Analysis using a CAT expression vector plasmid under the control of the ADF/Trx gene promoter revealed that CAT gene expression in Jurkat cells was increased after exposure to oxidative agents. A series of deletion analyses showed that a region from −976 to −890 of the 5′ flanking sequence was required for enhancement of ADF/Trx promoter activity against the oxidative agents. Gel mobility shift assay revealed the specific DNA binding activities to the sequences from −953 to −930 in the nuclear extracts from the Jurkat cells. The sequences in this region showed no homology with any known consensus sequences for DNA binding factors. It is suggested that ADF/Trx gene expression is enhanced through a novel c/s-acting regulatory element responsive for the oxidative stress and a new factor(s) is involved in this oxidative stress responsive element.