A photomultiplier tube assembly for the detection of low light levels
- 1 July 1983
- journal article
- instruments and-techniques
- Published by Springer Nature in Pflügers Archiv - European Journal of Physiology
- Vol. 398 (2) , 165-168
- https://doi.org/10.1007/bf00581066
Abstract
Bioluminescent indicators have found many uses in, for example, the detection of ATP and free calcium levels. However, such probes often emit only very low levels of light and it is important to optimise the efficiency of the system used to detect this light. We describe some of the problems encountered in using photomultiplier tubes for detecting low levels of light, and some ways of overcoming these problems. We have developed a versatile photomultiplier light detection system which is both efficient and physically small. This system is described and details of its fabrication are given.Keywords
This publication has 3 references indexed in Scilit:
- The effects of changes of pH on intracellular calcium transients in mammalian cardiac muscle.The Journal of Physiology, 1983
- Calcium transients in isolated amphibian skeletal muscle fibres: detection with aequorin.The Journal of Physiology, 1978
- On the Use of A Photomultiplier As A Photon CounterMonthly Notices of the Royal Astronomical Society, 1967