Serial Propagation in vitro of Agents Producing Inclusion Bodies Derived from Varicella and Herpes Zoster.

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Abstract
Roller tube cultures of human embryonic skin and muscle tissue or of human foreskin tissue were inoculated with vesicle fluid obtained from patients with varicella or herpes zoster. From the varicella materials 6 cytopathogenic agents were isolated. Histologically the lesions produced consisted of focal accumulations of swollen cells which subsequently degenerated; characteristically such swollen cells contained intranuclear inclusion bodies. Two of the strains were maintained for 10 tissue culture passages by employing tissue suspensions as the passage material. From the eruptive lesions of 2 cases of herpes zoster, cytopathogenic agents of a similar nature were isolated and propagated serially in cultures of human tissue.