Semi‐continuous culture and monitoring system for temperature‐synchronized Euglena

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Abstract
A temperature‐synchronized, semi‐continuous culture and monitoring system is described, with results from use of the system for autotrophically growing Euglena. Outflow from the culture vessel consists of measured samples taken automatically at 2‐hour intervals and fixed for later counting. Inflow is by siphon feed, which restores the culture level after each sampling. The interpretation of growth curves obtained from such cultures is discussed from the viewpoint, of division synchrony and cell cycle studies, and some general comparisons are made between batch and continuous cultures for such studies.