Monoclonal Antibodies Reveal Complex Structure in the Membrane Skeleton of Tetrahymena
- 1 July 1995
- journal article
- Published by Wiley in The Journal of Eukaryotic Microbiology
- Vol. 42 (4) , 422-427
- https://doi.org/10.1111/j.1550-7408.1995.tb01606.x
Abstract
Twelve monoclonal antibodies were raised that are specific for the membrane skeleton of Tetrahymena. Five were directed against T. pyriformis and seven were directed against T. thermophila. Some cross-reactivity between species was found. Each monoclonal antibody recognized one of the three major components of epiplasm, i.e. the bands A, B, and C identified in electrophoretic separations of epiplasmic proteins. It was found, using these antibodies, that the epiplasmic proteins A, B and C have overlapping but independent distributions within the cell.Keywords
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