[14C]-Aflatoxin B1 Metabolism in Lactating Goats and Rats

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Abstract
Four dairy goats in the second to third month of lactation were administered [14C]-Aflatoxin B1 ([14C] -AFB1). Two goats were dosed intravenously (iv) with 130 µCi (182 µCi/µmol) and two were dosed orally with 196 µCi (256 µCi/µmol). Urine, milk and feces were collected for 120 h after [14C]-AFB1 administration. Recoveries (average of two animals) of 14C in urine, milk and feces were, respectively; 22.7, .97 and 65% (iv dose) and 30.9, 1.05 and 52.3% (oral dose). Aflatoxin M1 (AFM1) was found in milk in the highest concentration. Aflatoxin Q1 (AFQ1) and aflatoxicol (AFL) were found in trace quantities in milk of animals administered the oral dose. In general, AFM1 could account for the majority of dicholromethane-soluble 14C. Liver contained 7.3 and 4.9% of the dose after 120 h for the iv and oral doses respectively. Kidney, heart, lung and spleen all contained .1% or less of the dose at 120 h. Muscle contained .48% of the dose at 120 h from the goats administered [14C]-AFB1 orally. There was no detectable radioactivity in the fat of any goat at 120 h. Six lactating Sprague-Dawley rats with 12 nursing pups were used for comparison of ruminants and simple-stomached animals. Rats were administered 2 µCi of [14C]-AFB1 (125 µCi/µmol) iv (three animals) and orally (three animals). Mean recovery of 14C in urine, mammary plus milk and feces were, respectively; 9.5, 2.0 and 60.7% (iv) and 8.8, 2.6 and 65.0% (orally). Excretion rates in lactating rats and goats are similar, although the lactating goat appears to have greater conjugating capacity as indicated by a greater percentage of dose in the goat's urine. Copyright © 1986. American Society of Animal Science . Copyright 1986 by American Society of Animal Science.

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