Multilabeled 13C substrates as probes in In vivo13C and 1H NMR spectroscopy
- 1 December 1989
- journal article
- research article
- Published by Wiley in NMR in Biomedicine
- Vol. 2 (5-6) , 274-277
- https://doi.org/10.1002/nbm.1940020516
Abstract
The potential use of 13C multilabeled substrates has been studied in biological applications using in vivo and in vitro proton and 13C NMR spectroscopy. In 13C NMR spectroscopy, multilabeled compounds allow the simultaneous observation of several nuclei or increase distinctly the signal to noise ratio due to a higher degree of enrichment. Contiguous labeling of substrates leads to homonuclear 13C‐13C spin couplings and provides a simple means to distinguish between endogenous stores of metabolites and metabolites derived from added substrates.Keywords
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