Expression of Platelet‐Activating Factor Receptor Transcript‐1 but Not Transcript‐2 by Human Bone Marrow Cells

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Abstract
The presence of platelet‐activating factor receptor (PAF‐R) transcripts 1 and 2 was investigated in human bone marrow cells by a reverse transcriptase polymerase chain reaction (RT‐PCR) procedure which detected their simultaneous presence. RT‐PCR experiments reveal PAF‐R transcript 1 (but not 2) in freshly isolated mononuclear marrow cells, CD34+ hematopoietic stem/progenitor cells and cultured marrow stromal cells. For these experiments, the 5637 human bladder carcinoma cell line is used as a positive control for the presence of PAF‐R transcripts 1 and 2. Flow cytometry experiments confirm the presence of PAF‐R on marrow stromal cells and CD34+ stem/progenitor cells. In conclusion, the expression of PAF‐R transcript 1, which mainly exists in circulating leukocytes, is also found in CD34+ stem/progenitor cells and cells of the marrow microenvironment, strengthening the potential role of PAF during marrow hematopoiesis.